Calibrated for Quantitative Accuracy
Our ORCA-Flash4.0 cameras have always provided the advantage of low camera noise. In quantitative applications, like single molecule imaging and super resolution microscopy imaging, fully understanding camera noise is also important. Every ORCA-Flash4.0 V3 is carefully calibrated… as a precision instrument must be. Our attention to this detail delivers outstanding linearity, especially at low light, and offers improved photo response non-uniformity (PRNU) and dark signal non-uniformity (DSNU) to minimize pixel differences and reduce fixed pattern noise. Each camera ships with a certificate providing the read noise and photoelectron conversion factor specific for that camera.
Flexibility for Customized Data Control
Like its predecessors, each ORCA-Flash4.0 V3 is capable of both USB 3.0 or Camera Link output. In addition, the ORCAFlash4.0 V3 offers data reduction through user-controllable look up tables (LUT) for 12 or 8-bit output. These two choices, combined with region of interest selection enable you to fine tune acquisition speed and image data requirements.
Patented Tools for Advanced Imaging
The ORCA-Flash4.0 V3 includes our now patented, Lightsheet Readout Mode which takes advantage of sCMOS rolling shutter readout to enhance the quality of lightsheet images.
When paired with our W-VIEW GEMINI image splitting optics, a single ORCA-Flash4.0 V3 camera becomes a powerful dual wavelength imaging device. In “W-VIEW Mode,” each half of the sensor can be exposed independently, facilitating balanced dual color imaging with a single camera. And this feature can be combined with the new and patented “Dual Lightsheet Mode” making simultaneous dual wavelength lightsheet microscopy a reality. And finally, the ORCA-Flash4.0 V3 is the perfect complement to our new W-VIEW GEMINI-2C dual camera, super resolution-quality, image splitting optics.
Focus on the Relevant Data
Our new “Enhanced Visualization Mode” was designed to help answer the question “Can I see it?” Many low light experiments, especially those that were previously imaged using EM-CCDs are now routinely accomplished—with better signal to noise, faster speeds and at far less expense—by sCMOS cameras. However, we sometimes miss the visual punchiness of EM-CCD images. Enhanced Visualization Mode pops the contrast of the displayed image, while
saving the sCMOS raw image data to disk.
And sometimes our innate tendency to seek contrast can be a distraction. Even a few hot pixels in a time lapse or tracking experiment can divert our attention or our analysis from the real data. Enter our multi-level, user-selectable, hot pixel reduction. By applying a series of increasingly aggressive algorithms to detect noisy pixels, you can choose to eliminate these small but distracting elements. And since nobody appreciates having their data unexpectedly modified, the default setting for this hot pixel correction is off when you power up the camera.
Powerful Triggering for Synchronization
Joining a full complement of sophisticated triggering routines, our new Master Pulse timing generator allows the ORCA-Flash4.0 V3 to truly run the experiment. Performing functions that previously would have required an external pulse generator the ORCA-Flash4.0 V3’s Master Pulse has flexible timing delays built in. Powerful synchronization of multiple cameras and devices just became a lot simpler.
|Quantum efficency||82 % (peak QE: @560 nm)|
|Effective no. of pixels||2048 (H)×2048 (V)|
|Cell size||6.5 μm×6.5 μm|
|Effective area||13.312 mm×13.312 mm|
|Full well capacity||30 000 electrons (typ.) *|
|Readout speed||100 frames/s (Full resolution, standard scan, Camera Link) *
40 frames/s (Full resolution, standard scan, USB 3.0, 16 bit) *
53 frames/s (Full resolution, standard scan, USB 3.0, 12 bit) *
80 frames/s (Full resolution, standard scan, USB 3.0, 8 bit) *
|Readout noise||Standard scan (at 100 frames/s, typ.):1.6 electrons rms (1.0 electrons median)
Slow scan (at 30 frames/s, typ.): 1.4 electrons rms (0.8 electrons median)
|Cooling method||Peltier cooling|
|Cooling temperature||Forced air (Ambient at +20 ℃): -10 ℃
Water (+20 ℃): -10 ℃
Water (+15 ℃): -30 ℃
|Dark current||0.06 electrons/pixel/s (Air Cooled to -10° C) (typ.)
0.06 electrons/pixel/s (Water Cooled to -10° C) (typ.)
0.006 electrons/pixel/s (Water Cooled to -30° C) (typ.)
|Dynamic range||37 000:1 (typ.)|
|Interface||Camera Link / USB 3.0|
|A/D converter||16 bit / 12 bit / 8 bit|
|Readout Modes||Normal Area, Lightsheet, W-VIEW Mode, Dual Lightsheet|
|Master Pulse Generator (Pulse Modes)||Internal Sync, Start Trigger, Burst|
|Master Pulse Generator (Pulse Interval in 1 μs increments)||10 μs to 10 s|
|Hot Pixel Correction||Off, Low, Medium, High|
|Dark Signal Non-Uniformity (DSNU)*||0.3 electrons rms|
|Photo Response Non-Uniformity (PRNU) at half level of full light range (15,000 electrons)*||0.06 % rms|
|Photo Response Non-Uniformity (PRNU) at low light level (700 electrons)*||0.3 % rms|
|Linearity error, full light range (EMVA 1288 standard)*||0.5 %|
|Linearity error, low light range (< 500 electrons signal)*||0.2 % / Less than approx. 1 electron absolute error|
|On-camera Connectivity||Both USB 3.0 and Camera Link**|
|V2 Compatibility Mode (for use with legacy software)||Yes|